B-ALL - Slide 2
A slide from a patient with an acute B-cell leukemia, which exhibits lymphoid blasts with a wide variety of shapes and sizes. The nuclei have a very fine chromatin pattern and some cells show nucleoli. The total leukocyte count was "only"
15⋅109/L
. Flowcytometry shows that there is also a small population of mature plasma cells present (CD38+, CD138+).
Blasts
Myelocytes
Bands
Segments
Eosinophils
Lymphocytes
Monocytes
Erythroblasts
Immunophenotype
CD19+
CD34+
HLA-DR+
TdT+
CD10+
CD20+
CD22+
cyCD79a+
CD66c+
A large population of CD19+, cyCD79a+, CD10+, CD22+, cyTdT+ B-lymphoblasts. The abnormal population is predominantly CD34 positive and heterogenously positive for CD20. In addition, the malignant cells (aberrantly) express the myeloid marker CD66c.
SSC
CD19
CD133
CD34
CD22
HLA-DR
CD20
CD10
cyCD79a
MPO
CD33
CD66c
Peripheral blood
Bone marrow
Blasts
Plasmacytoid lymphocytes
